Similarity of ribosomal and ribosomal precursor RNA's from rat liver and the Novikoff ascites hepatoma.
نویسندگان
چکیده
rat liver and the Novikoff hepatoma have been examined by two competitive hybridization techniques and by nucleotide analyses. Significant differences were not found between the corresponding RNA of these tissues by competition of labeled RNA from the hepatoma with unlabeled 18 and 28 S ribosomal RNA and 28 and 45 S nucleolar RNA of liver and hepatoma by sequential or simultaneous competition hybridization. Differences were found in the specific activity of 32P-labeled nucleotides, but no significant differences
منابع مشابه
Similarity of Ribosomal and Ribosomal Precursor RNA's from Rat Liver and the Novikoff Ascites Hepatoma1
rat liver and the Novikoff hepatoma have been examined by two competitive hybridization techniques and by nucleotide analyses. Significant differences were not found between the corresponding RNA of these tissues by competition of labeled RNA from the hepatoma with unlabeled 18 and 28 S ribosomal RNA and 28 and 45 S nucleolar RNA of liver and hepatoma by sequential or simultaneous competition h...
متن کاملA comparison of polypyrimidine fragments in 28 S ribosomal RNA from rat liver and the Novikoff ascites hepatoma.
The distributions of polypyrimidine sequences have been compared for ribosomal 28 S RNA from the Novikoff hepatoma and normal rat liver. Complete digestion at adenylyl and guanylyl residues by successive treatment with U2 RNase followed by TI RNase released oligonucleotides from highly labeled ribosomaJ RNA. These oligonucleotides were isolated by chromatography according to chain length on die...
متن کاملA Comparison of Polypyrimidine Fragments in 28 S Ribosomal RNA from Rat Liver and the Novikoff Ascites Hepatoma1
The distributions of polypyrimidine sequences have been compared for ribosomal 28 S RNA from the Novikoff hepatoma and normal rat liver. Complete digestion at adenylyl and guanylyl residues by successive treatment with U2 RNase followed by TI RNase released oligonucleotides from highly labeled ribosomaJ RNA. These oligonucleotides were isolated by chromatography according to chain length on die...
متن کامل;!"P Distribution in Oligonucleotides of 28 S Ribosomal RNA of the Novikoff Hepatoma and Normal Rat Liver1
35 S, and 28 S RNA of tumors is characterized by a lower adenylic acid content and a higher cytidylic acid content than that of nontumor tissues (3). In addition, it was found that the isotope content of the dinucleotides GC and GU of hepatomas was significantly higher than that of normal liver (18). For determination of whether these differences represented isotope effects or seg mentai differ...
متن کاملQuantitative determination of the ribosomal ribonucleic acid content of liver and Novikoff hepatoma from fed and from fasted rats.
Although never demonstrated unequivocally, it has been generally accepted that the largest fraction of the ribonucleic acid of mammalian cells is ribosomal. However, it has been reported recently that about 65% of rat liver RNA has a nonribosomal sedimentation coefficient in the range of 4 S. To resolve the question of the ribosomal RNA content of rat liver and to examine the validity of the as...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cancer research
دوره 33 12 شماره
صفحات -
تاریخ انتشار 1973